Coding

Part:BBa_K4633003:Design

Designed by: Neta Segal   Group: iGEM23_Technion-Israel   (2023-09-07)


araR - Bacillus subtilis repressor protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 119
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 146


Design Notes

The araR protein was assessed in B. subtilis 168, and the testing utilized composite part.


Source

The genetic sequence for the araR protein from the genome of B. subtilis 168 was obtained from the NCBI [1].

References

[1] B.subtilis araR and araE genes - Nucleotide - NCBI. https://www.ncbi.nlm.nih.gov/nuccore/X98354.1?from=874&to=1962#sequence_X98354.1